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wild type t denticola  (ATCC)


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    Structured Review

    ATCC wild type t denticola
    Wild Type T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+denticola/Treponema+denticola/pm41813854-135-11-14
    Average 96 stars, based on 899 article reviews
    wild type t denticola - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Periodontitis Progression During Cardiac Rehabilitation After Acute Coronary Syndrome: Cohort Study.
    Article Snippet: Objectives: To assess changes in periodontal status and factors related to periodontitis progression in acute coronary syndrome patients who undergo a cardiovascular rehabilitation program following an acute coronary incident.. Materials and Methods: Fiftytwo acute coronary syndrome patients with periodontitis were evaluated during a sixmonth followup period.. Periodontal indices were taken at baseline and at 3 and 6 months.

    Bacteria:

    Article Title: Periodontitis Progression During Cardiac Rehabilitation After Acute Coronary Syndrome: Cohort Study.
    Article Snippet: Objectives: To assess changes in periodontal status and factors related to periodontitis progression in acute coronary syndrome patients who undergo a cardiovascular rehabilitation program following an acute coronary incident.. Materials and Methods: Fiftytwo acute coronary syndrome patients with periodontitis were evaluated during a sixmonth followup period.. Periodontal indices were taken at baseline and at 3 and 6 months.

    In Silico:

    Article Title: In Silico Design and Evaluation of a Novel Multi-Epitope Vaccine Against Treponema denticola (strain ATCC 35405): A Reverse Vaccinology Approach
    Article Snippet: .. 34,35,52 The current study, up to our knowledge, is the first in silico based vaccinology study conducted against T. denticola (strain ATCC 35405) and we believe that it could provide an attractive alternative approach to tackle the dissemination of T. denticola related infections. ..

    Control:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 29 , Börnigen et al. 2017, Case control study, USA [ ] , 121 OSCC patients; 242 controls , Oral rinse sample , Microbiome analysis; 16 S rRNA gene sequencing , Not given , NA , Periodontal microorganisms not detected ( Treponema denticola , Porphyromonas gingivalis , Tannerella forsythensis ,) , OSCC. .. 30 , Kang et al. 2009, case control study, Korea [ ] , 104 oncological ( head and neck cancer , haematological neoplasia, solid tumours) patients and 52 healthy , Salivary samples , Microbial analysis using polymerase chain reaction , Not given , NA , T. denticola (ATCC 35405) was detected in 64.5% of patients with head and neck tumours and of these T. denticola (41.3%) was present in patients with haematological neoplasia and 71.1% in patients with solid tumours , HNSCC , haematological neoplasia and other tumours. .. 31 , Na et al. 2013, Cross sectional study, Korea [ ] , 36 subjects including 16 healthy volunteers, 11 periodontitis patients, and 9 OSCC patients , Gargling samples , PCR , Not given , NA , More in OSCC patients - Streptococcus salivarius , Capnocytophaga ochracea and Gemella morbillorum . Less in OSCC - Prevotella nigrescens , Fusobacterium nucleatum . Periodontitis patients- Porphyromonas gingivalis , Treponema denticola . , OSCC.

    Polymerase Chain Reaction:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 29 , Börnigen et al. 2017, Case control study, USA [ ] , 121 OSCC patients; 242 controls , Oral rinse sample , Microbiome analysis; 16 S rRNA gene sequencing , Not given , NA , Periodontal microorganisms not detected ( Treponema denticola , Porphyromonas gingivalis , Tannerella forsythensis ,) , OSCC. .. 30 , Kang et al. 2009, case control study, Korea [ ] , 104 oncological ( head and neck cancer , haematological neoplasia, solid tumours) patients and 52 healthy , Salivary samples , Microbial analysis using polymerase chain reaction , Not given , NA , T. denticola (ATCC 35405) was detected in 64.5% of patients with head and neck tumours and of these T. denticola (41.3%) was present in patients with haematological neoplasia and 71.1% in patients with solid tumours , HNSCC , haematological neoplasia and other tumours. .. 31 , Na et al. 2013, Cross sectional study, Korea [ ] , 36 subjects including 16 healthy volunteers, 11 periodontitis patients, and 9 OSCC patients , Gargling samples , PCR , Not given , NA , More in OSCC patients - Streptococcus salivarius , Capnocytophaga ochracea and Gemella morbillorum . Less in OSCC - Prevotella nigrescens , Fusobacterium nucleatum . Periodontitis patients- Porphyromonas gingivalis , Treponema denticola . , OSCC.

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    In Vitro:

    Article Title: Identification of cannabinoid-sensitive and -resistant oral bacteria
    Article Snippet: .. We have previously shown that multiple strains of T. denticola (ATCC 35404, 35405, 33520 and 33521) as well as Treponema putidum ATCC 700334 are resistant to CBD in an in vitro setting ( ). .. We have also reported that growth of the type strain of P. gingivalis (ATCC 33277) is abrogated upon phytocannabinoid exposure ( ).

    Proliferation Assay:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    Transmission Assay:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    Electron Microscopy:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    Flow Cytometry:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    Sequencing:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..

    Expressing:

    Article Title: The potential role of genus Treponema in carcinogenesis with a focus on oral squamous cell carcinoma: a scoping review of the evidence
    Article Snippet: 3 , Jones et al. 2019, USA [ ] , T. denticola (strain 35405, 33520, OTK) culture, murine neutrophils/ Enzyme linked immunosorbent assay, qRT PCR, immunoprecipitation , - , Msp , Msp manipulates neutrophil chemotaxis by signalling it. It impairs the immune response, which can contribute to chronic inflammation that is a risk factor for cancer development , Role in general carcinogenesis. .. 4 , Peng et al. 2022, China [ ] , OSCC cell line Cal-27 (ATCC CRL-1628)/ Cell proliferation assay, tumourigenesis assay, Transmission electron microscopy, flow cytometry, sequencing, PCR , T. denticola (ATCC 35405) could trigger TGF-β1, -β2, and -β3 mRNA expressions, affecting cell cycle regulation and cell proliferation. It also increased TLR4 and NF-kB expression. , - , T. denticola invasion regulated the cell cycle by allowing OSCC cell proliferation and inhibiting apoptosis. The spirochete enhanced TGF-β expression by activating TLR4-MyD88–NF-kB signaling. , OSCC. ..



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    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: <t>Treponema</t> <t>denticola</t> NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).
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    ATCC t denticola atcc 35405 protein sequence database
    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: <t>Treponema</t> <t>denticola</t> NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).
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    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: <t>Treponema</t> <t>denticola</t> NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).
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    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: <t>Treponema</t> <t>denticola</t> NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).
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    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: <t>Treponema</t> <t>denticola</t> NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).
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    ATCC t denticola
    Synergistic antimicrobial activity of CBD and select antimicrobial peptoids against T. <t>denticola</t> . (A) T. denticola ATCC 35405 was grown anaerobically in the presence or absence of peptoids A, B, D, E, or F (at mean IC 50 concentration; ) and CBD (5 μg/mL). Growth was monitored spectrophotometrically and cell density (O. D. 600 nm) plotted after 96 h. Structure of cathelicidin-mimicking peptoid B (B) and peptoid E (C) , respectively. The structures of peptoid B (MXB-2) and peptoid E (MXB-5) were first described in .
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    Image Search Results


    (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: Treponema denticola NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).

    Journal: bioRxiv

    Article Title: Bacterial exo-α-sialidases subvert the complement system through desialylation

    doi: 10.64898/2026.02.05.703967

    Figure Lengend Snippet: (a) Maximum-likelihood phylogenetic tree of bacterial GH33 exo-sialidases. A total of 289 sequences from UniProt and GenBank were aligned using MUSCLE, and a FastTree-based phylogeny was consolidated to 162 representative sequences spanning 55 bacterial genera. The scale bar indicates 0.2 amino acid substitutions per site. Experimentally characterized sialidases are highlighted in red: Treponema denticola NanH ( Td -NanH), Clostridium perfringens NanI ( Cp -NanI) and NanH ( Cp -NanH), Bacteroides fragilis NanH2 ( Bf -NanH2), Tannerella forsythia NanH ( Tf -NanH), and Vibrio cholerae NanH ( Vc -NanH). Tip symbols indicate distinct gene annotations. (b) Domain organization of the six sialidases. Amino acid positions are shown relative to the N-terminus. Domains were annotated using NCBI and CAZy resources. Signal peptides (SignalP-6.0) are shown in yellow, GH33 catalytic domains in red, and additional carbohydrate-binding modules (CBMs) or the pfam09479 domain in other colors. (c) Multiple sequence alignment of the conserved catalytic region containing the “Y/FRIP” motif. Secondary structure elements derived from Td -NanH are shown above the alignment, with the conserved “Y/FRIP” motif and Asp-box sequences highlighted. (d–h) Structural superposition of GH33 sialidases using Td -NanH (green) as the reference, overlaid with Cp -NanI (yellow; PDB 5TSP), Cp -NanH (salmon; PDB 8UB5), Bf -NanH2 (gray; AlphaFold), Tf -NanH (marine; PDB 7QYP), and Vc -NanH (magenta; PDB 1KIT).

    Article Snippet: T. denticola ATCC 35405 (wild type, WT) was used in this study .

    Techniques: Binding Assay, Sequencing, Derivative Assay

    Synergistic antimicrobial activity of CBD and select antimicrobial peptoids against T. denticola . (A) T. denticola ATCC 35405 was grown anaerobically in the presence or absence of peptoids A, B, D, E, or F (at mean IC 50 concentration; ) and CBD (5 μg/mL). Growth was monitored spectrophotometrically and cell density (O. D. 600 nm) plotted after 96 h. Structure of cathelicidin-mimicking peptoid B (B) and peptoid E (C) , respectively. The structures of peptoid B (MXB-2) and peptoid E (MXB-5) were first described in .

    Journal: Frontiers in Microbiology

    Article Title: Identification of cannabinoid-sensitive and -resistant oral bacteria

    doi: 10.3389/fmicb.2025.1709243

    Figure Lengend Snippet: Synergistic antimicrobial activity of CBD and select antimicrobial peptoids against T. denticola . (A) T. denticola ATCC 35405 was grown anaerobically in the presence or absence of peptoids A, B, D, E, or F (at mean IC 50 concentration; ) and CBD (5 μg/mL). Growth was monitored spectrophotometrically and cell density (O. D. 600 nm) plotted after 96 h. Structure of cathelicidin-mimicking peptoid B (B) and peptoid E (C) , respectively. The structures of peptoid B (MXB-2) and peptoid E (MXB-5) were first described in .

    Article Snippet: We have previously shown that multiple strains of T. denticola (ATCC 35404, 35405, 33520 and 33521) as well as Treponema putidum ATCC 700334 are resistant to CBD in an in vitro setting ( ).

    Techniques: Activity Assay, Concentration Assay

    CBD and antimicrobial peptoids induce ultrastructural abnormalities in sensitive oral bacteria. CBD, which is toxic to P. gingivalis , induced electron dense granular structures (black arrows) within the cytoplasm of this bacterium (A) [control] and (B) [CBD, 0.2 μg/mL]. CBD-treated cells exhibited less well-defined cell wall ultrastructure than their control counterparts (white bars) and were smaller. The presence of ghost cells was also noted (gray bars). Peptoid B treatment (14 μg/mL) induced clear morphological changes in T. denticola . Control cells (C) appeared intact with clear periplasmic flagella (black arrows), whereas peptoid-exposed T. denticola cells (D) appeared more translucent (white arrow), with exposed flagella (black arrows), indicative of a compromised outer membrane structure. Peptoid E-treated P. gingivalis 12 μg/mL (F) exhibited a more diffuse surface (white arrows) than untreated control cells (E) with ghost cells of this Gram-negative pathobiont also visualized (gray arrows). Peptoid E-treated cells were also smaller. The scale bars on panels A and B represent 600 nm. The bars on panels C , D , and F represent 400 nm. The bar on panel E represents 200 nm.

    Journal: Frontiers in Microbiology

    Article Title: Identification of cannabinoid-sensitive and -resistant oral bacteria

    doi: 10.3389/fmicb.2025.1709243

    Figure Lengend Snippet: CBD and antimicrobial peptoids induce ultrastructural abnormalities in sensitive oral bacteria. CBD, which is toxic to P. gingivalis , induced electron dense granular structures (black arrows) within the cytoplasm of this bacterium (A) [control] and (B) [CBD, 0.2 μg/mL]. CBD-treated cells exhibited less well-defined cell wall ultrastructure than their control counterparts (white bars) and were smaller. The presence of ghost cells was also noted (gray bars). Peptoid B treatment (14 μg/mL) induced clear morphological changes in T. denticola . Control cells (C) appeared intact with clear periplasmic flagella (black arrows), whereas peptoid-exposed T. denticola cells (D) appeared more translucent (white arrow), with exposed flagella (black arrows), indicative of a compromised outer membrane structure. Peptoid E-treated P. gingivalis 12 μg/mL (F) exhibited a more diffuse surface (white arrows) than untreated control cells (E) with ghost cells of this Gram-negative pathobiont also visualized (gray arrows). Peptoid E-treated cells were also smaller. The scale bars on panels A and B represent 600 nm. The bars on panels C , D , and F represent 400 nm. The bar on panel E represents 200 nm.

    Article Snippet: We have previously shown that multiple strains of T. denticola (ATCC 35404, 35405, 33520 and 33521) as well as Treponema putidum ATCC 700334 are resistant to CBD in an in vitro setting ( ).

    Techniques: Bacteria, Control, Membrane